Regulatory

Part:BBa_K3222000:Design

Designed by: Ryo NIWA   Group: iGEM19_GIFU_TOKAI   (2019-10-16)


T7 promoter with GGG of 3' end


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Removal of Sap1 restriction site was performed by silent mutation of 15A to 15G.


Source

Pedelacq et al. (2006): "Engineering and characterization of a super folder green fluorescent protein", Nature Biotech 24 (1) January 2006 See the detailed information of https://parts.igem.org/Part:BBa_I746916

References